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Chemical Name:TRIS base
Molecular Formula:C4H11NO3
Formula Weight:121.14
CAS No.:77-86-1
Chemical Name:TRIS base
Molecular Formula:C4H11NO3
Formula Weight:121.14
CAS No.:77-86-1
Appearance: White Powder
Assay: 99%min
Tris(hydroxymethyl)aminoethane / CAS:77-86-1 Typical Properties
Item | Specifications |
Appearance | White crystal powder |
Assay | 99.5% min |
Solubility(40% aq.) | Clear, colorless, solution |
Moisture | 0.5% max |
PH value (5% aq.) | 10.5~12 |
Fe | 1 ppm max |
SO4 | 10 ppm max |
Cl | 3 ppm max |
Ultraviolet absorbance(40% aq.) | 0.07 max |
Ultraviolet absorbance(40% aq.) | 0.02 max |
Tris(hydroxymethyl)aminoethane / CAS:77-86-1 Application field
1. Tris is often used as a biological buffer with a pH of 6.8, 7.4, 8.0, 8.8.
2. Tris is widely used in the preparation of buffers for biochemical and molecular biology experiments. For example, tries are commonly used in TAE and TBE buffers (for the solubilization of nucleic acids) andin biochemical experiments. Since it contains an amino group, it can undergo a condensation reaction with an aldehyde.
3. Tris is a weak base, and its pKa is 8.1 at room temperature (at 25 ° C; according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2.
4. The pH of the aqueous solution of the Tris base is about 10.5. Usually, hydrochloric acid is added to adjust the pH to the desired value to obtain a buffer of the pH. But at the same time, attention should be paid to the effect of temperature on the pKa of Tris.
Tris(hydroxymethyl)aminoethane / CAS:77-86-1 Packaging
25kgs/drum
Tris(hydroxymethyl)aminoethane / CAS:77-86-1 Storage
Store in a cool, ventilated warehouse. Keep away from fire and heat sources. Keep the container sealed
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Other product:Resazurin sodium salt / CAS:62758-13-8
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